DOT Specimen Collector Training

National Drug Screening offers DOT Specimen Collector Training for both urine and oral fluid specimens. These are separate training programs. DOT qualification for urine and oral fluid both require proficiency demonstration or mock collections to complete the training and receive certification.

Specimen Collector Training Options

All of our training programs include a comprehensive training manual with links to video demonstration of the specimen collection process.

Oral Fluid Mock CollectionsUrine Specimen Mock Collections

For larger groups for live training or custom webinar training, please call Joe Reilly @ 321-622-2020.

What is required for DOT specimen collector training?

DOT specimen collector training requirements are outlined in 49 CFR Part 40 and include specific components to ensure collectors are properly prepared for their responsibilities. Below are the key requirements:

Qualification Training

  1. Content of Training:
    • Detailed instruction on the Part 40 collection procedures.
    • Steps for completing collections accurately, including using the Custody and Control Form (CCF).
    • Managing challenging situations such as tampering attempts, “shy bladder” “shy lung” insufficient specimen scenarios.
    • Identification and correction of fatal and correctable flaws.
    • Maintaining the integrity of the collection process and ensuring donor privacy​​.
  2. Device-Specific Training:
    • Proficiency in using the specific oral fluid or urine collection devices to be employed​​.


Proficiency Demonstration

  • Mock Collections:
    • Collectors must perform five consecutive error-free mock collections, covering various scenarios such as standard collection, insufficient quantity, tampering attempts, refusal to sign forms, etc..
    • These mock collections must be observed and evaluated by a qualified trainer who certifies the collector’s proficiency.


Error Correction Training

  • If a collector’s mistake leads to a canceled test, they must undergo retraining within 30 days.
  • This includes three additional mock collections focusing on the areas where the error occurred.


Refresher Training

  • Every five years, collectors must complete refresher training and re-demonstrate their proficiency to stay compliant.


Documentation and Certification

  • Collectors must maintain documentation of their training and proficiency, which must be made available to DOT representatives or employers upon request.


These comprehensive requirements are designed to ensure all DOT collectors are thoroughly prepared to conduct drug and alcohol tests according to the regulations.

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