DOT 5 Panel Drug Test

If you are employed by the Department of Transportation and are in need of a 5-panel drug test, we can help. Learn more about what drugs you will be tested for, cutoff levels and more from National Drug Screening.

What does the DOT drug testing panel look like with the new expanded Opiates now called Opioids? As specified in DOT regulations 49 CFR Part 40 with the Cut Off levels listed in DOT regulations 49 CFR Part 40; the following substances will be tested for:

Some common names for the new semi-synthetic opioids include OxyContin®, Percodan®, Percocet®, Vicodin®, Lortab®, Norco®, Dilaudid®, and Exalgo®.

More About DOT Drug Testing

DOT 5 Panel Drug Test Cut Off Levels

The chart below lists the five panel DOT drug test with the Cut Off levels.

Initial Test Analyte

Marijuana metabolites (THCA)

Cocaine Metabolites (Benzoylecgonine)

Phencyclidine (PCP)

Amphetamine

Methamphetamine

MDMA/MDA

Codeine/Morphine

6-Acetylmorphine (6AM or Heroin)


Hydrocodone

Hydromorphone

Oxymorphone

Oxycodone

Initial Test Cutoff Concentration

50 ng/mL

150 ng/mL


25 ng/mL

250 ng/mL

500 ng/mL

250 ng/mL.

500 ng/mL

10 ng/mL


300 ng/mL

300 ng/mL

100 ng/mL

100 ng/mL

Confirmatory Test Analyte

THCA

Benzoylecgonine


Phencyclidine (PCP)

Amphetamine

Methamphetamine

MDMA/MDA

Codeine/Morphine

6-Acetylmorphine (6AM or Heroin)

Hydrocodone

Hydromorphone

Oxymorphone

Oxycodone

Confirmatory test cutoff concentration

15 ng/mL

100 ng/mL

25 ng/mL

250 ng/mL

250 ng/mL

250 ng/mL

250 ng/mL

10 ng/mL


100 ng/mL

100 ng/mL

100 ng/mL

100 ng/mL

National Drug Screening provides great information on DOT drug & alcohol testing programs. These programs include written policies and random consortium management.

Visit our DOT Compliance page for more great information, or give us a call at 866-843-4545 and one of our knowledgable associates will be happy to assist you!