DOT-regulated drug testing is required for employees regulated by the Department of Transportation. National Drug Screening provides DOT drug testing and services to employers and individuals nationwide. All of our DOT drug and alcohol testing programs are DOT compliant and can easily be purchased online or by calling 866-843-4545. Easily choose from a one time test or enroll in a consortium program. Learn more about DOT Compliance for FAA regulated employers, owner operator truck drivers, private fleets, for hire carriers, and small trucking and bus companies.